Reversibly-assebmled Perfusion Culture Chip with Microwell Array for Controllable Spheroid Culture and Post-culture Analysis

This paper reports a microfluidic device composed of a reversibly-assembled microfluidic chamber chip and multi-microwell array chip. This device enabled perfusion culture of spheroid in a microarray format, and detailed post-culture analysis by collection of spheroids from the disassembled device. The device provides four advantages simultaneously; the uniform spheroid formation, growth analysis in a microarray format, controlled proliferation by flow rate of perfusion, and post-culture analysis by collection of spheroids from the disassembled device. Thus, this method is a promising tool for fundamental and application research in drug screening, tissue engineering, and regenerative medicine.